Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis.

Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis.

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BackgroundThe comparatively straightforward and cheaper light-emitting diode fluorescent microscope (LEDFM) was suggested by WHO to replace conventional microscope in tuberculosis (TB) laboratories.However, the comparable efficacy of each NEFF C27CS22H0B N90 Built In 60cm A+ Electric Single Oven Stainless Steel of those techniques differs from laboratory to laboratory.We investigated the efficacy of LEDFM for the diagnosis of tuberculous lymphadenitis (TBLN) patients.MethodsA cross-sectional study was conducted on 211 samples from clinically suspected tuberculous lymphadenitis patients.Three smears were prepared from FNA on microscope slides for cytomorphology study, Auramine O (AO), and for Ziehl-Neelsen (ZN) staining.

The left-over samples were inoculated onto Lowenstein-Jensen (LJ) media.Statistical analysis was done using STATA version 11.The sensitivity, specificity, positive and negative predictive values were calculated by considering the culture results as the gold standard using a 95% confidence interval.ResultsAmong 211 samples 49.7% Bath Brush (105) were positive by cytomorphology, 32.

7% (69) by LEDFM, 23.69% (50) by LJ culture, and 13.7% (29) by ZN.Compared to the gold standard sensitivity of ZN, LEDFM, and cytomorphology were 30% [95% CI: 17.9-44.

6], 66% [95% CI: 51.2-78.8] 78% [95% CI: 64-88.5], respectively.The specificity of ZN, LEDFM, and cytomorphology was 91.

3% [95% CI: 85.8-95.2], 77.6% [95% CI: 70.4-83.

8], 58.8% [95% CI: 50.7-66.5], respectively.ConclusionLED fluorescence microscopy gives a legitimate option in contrast to conventional ZN techniques in terms of its higher sensitivity, a bit lower specificity, time-saving, and minimal effort.